Estimating specimen size
In order to determine the size of a specimen through the microscope, you need to know three things:
- how large the specimen is in terms of divisions of the ocular micrometer
- at what magnification the specimen is being observed
- how large one division of the ocular micrometer is at that particular magnification.
Once you know these, you simply multiply #1 by #3 for an estimate of the size of the specimen.
Answers to questions
One division of ocular micrometer
| = 0.025 mm at 40 X = |
25 micrometers at 40 X |
| = 0.01 mm at 100 X = |
10 micrometers at 100 X |
| = 0.0025 mm at 400 X = |
2.5 micrometers at 400 X
|
| = 0.001 mm at 1000 X = |
1 micrometer at 1000 X |
These values are based on a field of view at 40X of 4mm.
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